January 31st, 2012 | Category: Arabidopsis, Papers, Video | Leave a comment

Deep see!

We’ve just published a JoVE protocol showing how to use perfluorodecalin to image plant leaves at depth. This is a simple video which summarises the technique used in our 2010 paper on infiltrative imaging.

We describe here the application of the perfluorocarbon, perfluorodecalin (PFD), as an infiltrative imaging medium which optically improves laser scanning confocal microscopy (LSCM) sample imaging at depth, without resorting to damaging increases in laser power and has minimal physiological impact

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